Laboratory Animal Core Facility

Office address:

Laboratory Animal Core Facility

Department of Biochemistry and Molecular Biology,

Research Center for Molecular Medicine

Medical and Health Science Center, University of Debrecen, Hungary

Life Science Building, Nagyerdei krt. 98. Debrecen, Hungary H-4012

Phone: +36-52-416 432/62335 (office), 65234 (lab), 62325(MD), 62326 (SPF)

Fax: +36-52-314 989

e-mail:  This email address is being protected from spambots. You need JavaScript enabled to view it.  or  This email address is being protected from spambots. You need JavaScript enabled to view it.

Web: http://bmbi.med.unideb.hu/joomla15/index.php/en/core-facilities


1. Mission

The core facility was founded in 2005 to provide a professional background of animal experiments and promote best practices of humane care, use and management of animals involved in medical research at the Debrecen University.

We advance the development and acceptance of high standards for the responsible use of laboratory animals and accurate and reproducible animal experiments.

The Laboratory Animal Core Facility is administratively responsible for all animal care resources and the purchase and care of all laboratory animals in the Life Science Centre.


2. Housing conditions and laboratory capabilities

The facility is located on the Debrecen University campus as a part of the Life Science Centre. Suitable housing conditions are provided for a wide variety of outbred, inbred and genetically modified laboratory animals as mice, rats, guinea pigs and invertebrate organisms.

The Laboratory Animal Core Facility operates a clinical pathology laboratory for the purpose of basic disease surveillance and diagnosis as well as serving as a research resource to investigators. The laboratory capabilities include histology, immunocytochemsitry, clinical chemistry, chromatography, bacteriology.

 

3. Welfare and quality control

Specialized animal housing spaces include a quarantine suite, infectious biohazard containment suites. All animal rooms and the operation rooms are ventilated with HEPA filtered air. Our technologies see the requirements of FELASA recommendations and DIN EN ISO 9001 standards.

 

4. Members of the facility

Management

Supervisor

Zsuzsanna Szondy, MD, DSc

Professor of the Faculty of Medicine

 

Facility manager

Attila Pap, MSc

Biologist

 

Advisors

Árpád Bacsadi DVM

Pathologist

 

Péter Keserű DVM

Veterinarian

 

Judit Szabó MD, PhD

Bacteriologist 

 

Technicians: 

Sándor Sipos

Certified laboratory animal technician (2012-)

A2 level animal technician (2007-)

A1 level animal technician (2005-2007)

Certified technician of laboratory chemistry (1972-) 

 

János Tóth

Certified laboratory animal technician (2012-)

A1 level animal technician (2006-)

Certified medical technician (1983-)

 

Viktória Kurucz

A1 level animal technician (2012-)

Certified medical assistant (1998-)

 

Szabolcs Szalóczi

A1 level animal technician (2012-)

 


HEALTH MONITORING PROGRAMME

Animals are housed separately by species and, when possible, by source and or microbiological status. All rodent colonies are housed and maintained under minimal specific pathogen free (SPF) conditions. In our SPF definition we are negative for the following animal pathogens:

 

 

Test

frequency

Sample

tested

Test

method

Viruses

     

Mouse hepatitis virus (MHV)

3 months

Blood serum

ELISA, IFA

Mouse rotavirus (EDIM)

3 months

Blood serum

ELISA

Minute virus of mice (MVM)

3 months

Blood serum

ELISA

Mouse parvovirus (MPV1+2)

3 months

Blood serum

ELISA

Pneumonia virus of mice (PVM)

3 months

Blood serum

ELISA

Sendai virus (SEND)

3 months

Blood serum

ELISA

GD-7 (Theiler’s murine

encephalomyelitis virus)

3 months

Blood serum

ELISA

Ectromelia virus (ECTRO)

Annually

Blood serum

ELISA

Lymphocytic choriomeningitis

virus (LCMV)

Annually

Blood serum

ELISA

Mouse adenovirus (MAD1+2)

Annually

Blood serum

ELISA

Mouse cytomegalovirus

(MCMV)

Annually

Blood serum

ELISA

Reovirus type 3 (REO-3)

Annually

Blood serum

ELISA

Murine norovirus (MNV)

3 months

Blood serum

ELISA, IFA

Bacteria,

mycoplasma and fungi

     

Clostridium piliforme (Tyzzer’s d.)

3 months

Blood serum

ELISA

Mycoplasma pulmonis (MPUL)

3 months

Blood serum

ELISA

Corynebacterium kutcheri

3 months

Colon

CULT

Citrobacter rodentium

3 months

Nasal, respiratory tract

CULT

Pasteurellaceae

3 months

Vagina, nasal, respiratory tract,

CULT

Salmonella spp.

3 months

Caecum, hepar

CULT

Streptococci β-haemolytic, Streptococcus pneumoniae

3 months

Nasal, respiratory tract

CULT

Staphylococcus aureus

3 months

Nasal, respiratory tract

CULT

Bordetella spp.

3 months

Nasal, respiratory tract

CULT

Klebsiella spp.

3 months

Nasal, respiratory tract

CULT

Helicobacter spp.

Annually

Faeces

IP

Streptobacillus moniliformis

3 months

Nasal, respiratory tract

CULT

Parasites

     

Ectoparasites

3 months

Skin

MICR

Endoparasites

3 months

Faeces, colon

MICR

Pathological lesions observed by necropsy

3 months

 

PATH/

HIST

 

Diagnostic laboratories:

  1. AnLab LTD, Videnska 1083, 142 20 Prague 4, Czech Republic, Phone: +420 261711667
  2. BioDoc, Feodor-Lynen-Strasse 23. D-30625 Hannover, Germany, Phone: +49 511-548885
  3. MGSzH Állategészségügyi Diagnosztikai Igazgatóság, 4031 Debrecen, Bornemissza u. 3-5., T:+36-52-418-133

 



AIR STERILITY

The animal rooms are ventilated with sterile filtered positive pressured (+10%) air. The exchange rate is 13.000 m3 air/hour (with tenfold ventilation). The sterility of air flow is tested monthly by sedimentation test and cultivation of sedimented bacteria.


LABORATORY ANIMAL STRAINS

MOUSE STRAINS

OUTBRED STRAINS

1. NMRI

INBRED STRAINS

1. BALB/c

2. C57 BL/ 6J

3. 129Sv

GENETICALLY MODIFIED STRAINS

1. Adora2 (-) (Tm)

2. Adora3 (-) (Tm)

3. Boy6J

4. CD11cCre (Tg)

5. Dicer (fl)/ LysMCre (Tm,Tg)

6. Nur77 (-) (Tm)

7. PARP1 (-) (Tm)

8. PARP2 (-) (Tm)

9. PPARg (fl) (Tm)

10. PPARg (fl)/ LysMCre (TmTg)

11. PPARg (fl)/ Sox2Cre (TmTg)

12. PSGL1 (-) (Tm)

13. RXRa (fl)/ RXRb (-) (Tm)

14. RXRa (fl)/ RXRb (-)/ LysCre (TmTg)

15. Sh3pxd2b (-) (Tm)

16. STAT1 (-) (Tm)

17. STAT6 (-) (Tm)

18. TGM2 (-) (Tm)

IMMUNODEFICIENT STRAINS

1. SCID

 

RAT

Wistar white

 

GUINEA PIG

Hartley

We do not maintain rat and guinea pig strains in the animal house, but we are able to do this. If somebody wants to use rats or guinea pigs, we could order the animals from proper vendors.

 

 

Price list (from 1st January 2014):

 

Animal maintenance (cage/day)

Species

UD

Academic and Spin-off

Industry

laboratory mouse

65 HUF

70 HUF

90 HUF

laboratory rat

195 HUF

215 HUF

285 HUF

guinea pig

390 HUF

430 HUF

590 HUF

Pregnant female (for embrio isolation)

Pregnant female mouse

UD

Academic and Spin-off

Industry

E10-14 days

6.590 HUF

7.200 HUF

9.150 HUF

E15-20 days

7.200 HUF

7.900 HUF

10.400 HUF

Mother+newborns

7.560 HUF

8.300 HUF

11.000 HUF

Maintenance of breeding cages

Breeding cage (cage/month)

UD

Academic and Spin-off

Industry

Mouse

3.300 HUF

3.660 HUF

5.000 HUF

Animals for experiments from our own breeding colonies

Inbred strains

UD

Academic and Spin-off

Industry

C57BL/6J and BALB/c

     

4 weeks

1.500 HUF

1.700 HUF

2.200 HUF

6 weeks

2.000 HUF

2.200 HUF

3.000 HUF

8 weeks

2.500 HUF

2.800 HUF

3.700 HUF

12 weeks

3.500 HUF

4.000 HUF

5.300 HUF

16 weeks

4.500 HUF

5.000 HUF

6.800 HUF

20 weeks

5.500 HUF

6.100 HUF

8.200 HUF

 

Outbred strain

UD

Academic and Spin-off

Industry

NMRI

     

4 weeks

640 HUF

730 HUF

980 HUF

6 weeks

800 HUF

940 HUF

1.350 HUF

8 weeks

1.000 HUF

1.200 HUF

1.600 HUF

12 weeks

1.400 HUF

1.600 HUF

2.100 HUF

16 weeks

1.700 HUF

2.000 HUF

2.700 HUF

20 weeks

2.100 HUF

2.400 HUF

3.700 HUF

 

Immunodeficient strains

UD

Academic and Spin-off

Industry

 SCID 

     

4 weeks

3.500 HUF

4.200 HUF

5.500 HUF

6 weeks

4.000 HUF

4.800 HUF

6.200 HUF

8 weeks

4.500 HUF

5.400 HUF

6.900 HUF

12 weeks

5.500 HUF

6.600 HUF

8.500 HUF

16 weeks

6.500 HUF

7.800 HUF

10.000 HUF

20 weeks

7.500 HUF

9.000 HUF

11.500 HUF

 

Services

Name

UD

Academic and Spin-off

Industry

Mouse marking

90 HUF/mouse

100

HUF/mouse

130 HUF/mouse

Genotiping PCR 1 reaction

2.000 HUF/sample

2.200

HUF/sample

3.500 HUF/sample

Genotiping PCR 1 reaction

3.000 HUF/sample

3.200

HUF/sample

4.500 HUF/sample

Genotiping PCR>2 reaction

3.000+1.000 HUF/sample

3.200+1.000 HUF/sample

4.500+1.000HUF/sample

Genotiping protocol setup

20.000 HUF

25.000 HUF

40.000 HUF

Oxymax/CLAMS usage

1.500 HUF/hour

2.000

HUF/hour

3.000 HUF/hour

Reflovet Plus usage

1.000 HUF/hour

1.300

HUF/hour

2.000 HUF/hour

 

Other orders, demands, experiments according to customized agreement.