Laboratory Animal Core Facility

Office address:

Laboratory Animal Core Facility

Department of Biochemistry and Molecular Biology,

Research Center for Molecular Medicine

Medical and Health Science Center, University of Debrecen, Hungary

Life Science Building, Nagyerdei krt. 98. Debrecen, Hungary H-4012

Phone: +36-52-416 432/62335 (office), 65234 (lab), 62325(MD), 62326 (SPF)

Fax: +36-52-314 989

e-mail:  This email address is being protected from spambots. You need JavaScript enabled to view it.  or  This email address is being protected from spambots. You need JavaScript enabled to view it.

Web: http://bmbi.med.unideb.hu/joomla15/index.php/en/core-facilities

1. Mission

The core facility was founded in 2005 to provide a professional background of animal experiments and promote best practices of humane care, use and management of animals involved in medical research at the Debrecen University.

We advance the development and acceptance of high standards for the responsible use of laboratory animals and accurate and reproducible animal experiments.

The Laboratory Animal Core Facility is administratively responsible for all animal care resources and the purchase and care of all laboratory animals in the Life Science Centre.

2. Housing conditions and laboratory capabilities

The facility is located on the Debrecen University campus as a part of the Life Science Centre. Suitable housing conditions are provided for a wide variety of outbred, inbred and genetically modified laboratory animals as mice, rats, guinea pigs and invertebrate organisms.

The Laboratory Animal Core Facility operates a clinical pathology laboratory for the purpose of basic disease surveillance and diagnosis as well as serving as a research resource to investigators. The laboratory capabilities include histology, immunocytochemsitry, clinical chemistry, chromatography, bacteriology.

3. Welfare and quality control

Specialized animal housing spaces include a quarantine suite, infectious biohazard containment suites. All animal rooms and the operation rooms are ventilated with HEPA filtered air. Our technologies see the requirements of FELASA recommendations and DIN EN ISO 9001 standards.

4. Members of the facility

Management

Supervisor

Zsuzsanna Szondy, MD, DSc

Professor of the Faculty of Medicine

Facility manager

Attila Pap, MSc

Biologist

Advisors

Árpád Bacsadi DVM

Pathologist

Péter Keserű DVM

Veterinarian

Judit Szabó MD, PhD

Bacteriologist 

Technicians: 

Sándor Sipos

Certified laboratory animal technician (2012-)

A2 level animal technician (2007-)

A1 level animal technician (2005-2007)

Certified technician of laboratory chemistry (1972-) 

János Tóth

Certified laboratory animal technician (2012-)

A1 level animal technician (2006-)

Certified medical technician (1983-)

Viktória Kurucz

A1 level animal technician (2012-)

Certified medical assistant (1998-)

Szabolcs Szalóczi

A1 level animal technician (2012-)

HEALTH MONITORING PROGRAMME

Animals are housed separately by species and, when possible, by source and or microbiological status. All rodent colonies are housed and maintained under minimal specific pathogen free (SPF) conditions. In our SPF definition we are negative for the following animal pathogens:

Diagnostic laboratories:

1. AnLab LTD, Videnska 1083, 142 20 Prague 4, Czech Republic, Phone: +420 261711667
2. BioDoc, Feodor-Lynen-Strasse 23. D-30625 Hannover, Germany, Phone: +49 511-548885
3. MGSzH Állategészségügyi Diagnosztikai Igazgatóság, 4031 Debrecen, Bornemissza u. 3-5., T:+36-52-418-133

AIR STERILITY

The animal rooms are ventilated with sterile filtered positive pressured (+10%) air. The exchange rate is 13.000 m3 air/hour (with tenfold ventilation). The sterility of air flow is tested monthly by sedimentation test and cultivation of sedimented bacteria.

LABORATORY ANIMAL STRAINS

MOUSE STRAINS

OUTBRED STRAINS

1. NMRI

INBRED STRAINS

1. BALB/c

2. C57 BL/ 6J

3. 129Sv

GENETICALLY MODIFIED STRAINS

1. Adora2 (-) (Tm)

2. Adora3 (-) (Tm)

3. Boy6J

4. CD11cCre (Tg)

5. Dicer (fl)/ LysMCre (Tm,Tg)

6. Nur77 (-) (Tm)

7. PARP1 (-) (Tm)

8. PARP2 (-) (Tm)

9. PPARg (fl) (Tm)

10. PPARg (fl)/ LysMCre (TmTg)

11. PPARg (fl)/ Sox2Cre (TmTg)

12. PSGL1 (-) (Tm)

13. RXRa (fl)/ RXRb (-) (Tm)

14. RXRa (fl)/ RXRb (-)/ LysCre (TmTg)

15. Sh3pxd2b (-) (Tm)

16. STAT1 (-) (Tm)

17. STAT6 (-) (Tm)

18. TGM2 (-) (Tm)

IMMUNODEFICIENT STRAINS

1. SCID

RAT

Wistar white

GUINEA PIG

Hartley

We do not maintain rat and guinea pig strains in the animal house, but we are able to do this. If somebody wants to use rats or guinea pigs, we could order the animals from proper vendors.

Price list (from 1st January 2014):

Animal maintenance (cage/day)

Pregnant female (for embrio isolation)

Maintenance of breeding cages

Animals for experiments from our own breeding colonies

Services

Other orders, demands, experiments according to customized agreement.