János Aradi
Current appointment(s):
Associate professor of Biochemistry and Molecular Biology,
Department of Biochemistry and Molecular Biology, University of Debrecen, Hungary (1991-)
CEO and Chief Scientific Officer of BIOMER Ltd. (2005-)
Contacts:
Office address:
Department of Biochemistry and Molecular Biology, Research Center for Molecular Medicine
Medical and Health Science Center, University of Debrecen, Hungary
Life Science Building, Egyetem tér 1., Debrecen, Hungary H-4010
Phone: +36-52-416432
Fax: +36-52-314989
General information:
Languages:
Hungarian (native)
English (State Exam, C Type, Intermediate Level #A3636/1979)
Russian (State Exam, Basic Level # A 001558/1988)
Academic background:
Enzymatic synthesis of polynucleotides containing thiolated pyrimidines and study their inhibitory activities on DNA polymerases.
Thesis for the degree of Candidate of Biological Science (biochemistry), Hungarian Academy of Sciences, Budapest , Hungary (1989)
Application of polynucleotide phosphorylase enzyme for directed synthesis of ribooligonucleotides.
Thesis for the degree of Med. Biol. Doctorate (biochemistry), University Medical School of Debrecen, Debrecen, Hungary (1973)
Study the stability of complexes of rare earth elements.
Thesis for the degree of M.Sc. (inorganic chemistry), Lajos Kossuth University of Art and Science, Debrecen, Hungary (1966)
Undergraduate student in Chemistry, Lajos Kossuth University of Art and Science, Debrecen, Hungary (1961-1965)
Scientific background:
Associate Professor of Biochemistry and Molecular Biology, Department of Biochemistry and Molecular Biology, University of Debrecen, Hungary, (1991-)
Assistant Professor of Biochemistry and Molecular Biology, Department of Biochemistry and Molecular Biology, University of Debrecen, Hungary, (1979-1991)
Senior Research Associate, Department of Medicinal Chemistry. State University of New York at Buffalo, Buffalo, NY, USA, (1987-1988)
Senior Research Associate, Department of Medicinal Chemistry. State University of New York at Buffalo, Buffalo, NY, USA, (1982-1983)
Postdoctoral Associate, Department of Medicinal Chemistry. State University of New York at Buffalo, Buffalo, NY, USA Advisor: Thomas J. Bardos, Ph.D. (1973-1974)
Assistant Lecturer of Biochemistry Department of Biochemistry, University Medical School of Debrecen, Hungary, (1966-1979)
Current memberships in professional societies:
Hungarian Biochemical Society, Member since 1975.
Hungarian Biological Society, Member since 1988.
Working Group of Nucleotide Chemistry, Hungarian Academy of Sciences, 1995.
Awards:
Lecturer Of The Year (1993)
Széchenyi Professorship (1996-1998)
Lecturer Of The Year (1997)
Excellence in Education (1999)
Széchenyi Istvan Fellowship (2000-2003)
Lecturer Of The Year (2002)
Diploma of Merit, donated by the Rector of University of Debrecen (2003)
University of Debrecen Award for Innovation (2004)
Publications:
2006
33. Kemény-Beke A, Berényi E, Facskó A, Damjanovich J, Horváth A, Bodnár A, Berta A, Aradi J. (2006) Antiproliferative Effect of 4-Thio-Uridylate on OCM-1 Uveal Melanoma Cells. Eur J Opthalmol. 16, 680-85.
32. Horváth A, Beck Z, Bardos TJ, Dunn JA, Aradi J. (2006) Effect of the extent of thiolation and introduction of phosphorothioate internucleotide linkages on the anti-HIV activity of Suligovir [(s4dU)35]. Bioorg Med Chem Lett. 16, 5321-5323.
31. Kemény-Beke Á, Aradi J, Damjanovich J, Beck Z, Facskó A, Berta A, Bodnár A. (2005) Apoptotic response of uveal melanoma cells upon treatment with chelidonine, sanguinarine and chelerythrine. Cancer Lett. 237, 67-75.
30. Katona K, Hercezegh P, Kappelmayer J, Fésüs L, Aradi J. (2006) Deoxy-adenosine-monophosphate di-n-butylester induces apoptosis by increasing the dATP level in HL-60 cells. Cancer Lett. 235, 281-90.
29. Wu Y, Dudognon C, Nguyen E, Hillion J, Pendino F, Tarkanyi I, Aradi J, Lanotte M, Tong J, Chen GQ, Ségal-Bendirdjian E. (2006) Immunodetection of human telomerase transcriptase (hTERT) re-appraised: nucleolin and telomerase cross paths. J Cell Sci. 119, 2797-806.
28. Pendino F, Tarkanyi I, Dudognon C, Hillion J, Lanotte M, Aradi J, Segal-Bendirdjian E. (2006) Telomeres and telomerase: pharmacological targets for new anticancer strategies? Curr Cancer Drug Targets. 6, 147-80.
2005
27. Tarkanyi I, Dudognon C, Hillion J, Pendino F, Lanotte M, Aradi J, Segal-Bendirdjian E. (2005) Retinoid/arsenic combination therapy of promyelocytic leukemia: induction of telomerase-dependent cell death. Leukemia. 19, 1806-11.
26. Horváth A, Tőkés S, Hartman T, Watson K, Turpin JA, Buckheit RW Jr, Sebestyén Z, Szöllősi J, Benkő I, Bardos TJ, Dunn JA, Fésüs L, Tóth FD, Aradi J. (2005) Potent inhibition of HIV-1 entry by (s4dU)35. Virology. 334, 214-23.
25. Horváth A, Aradi J. (2005) Advantages of sodium perchlorate solution as mobile phase for purification of synthetic oligonucleotides by anion exchange chromatography. Anal Biochem. 338, 341-3.
24. Tarkanyi I, Horváth A, Szatmari I, Eizert H, Vámosi G, Damjanovich S, Ségal-Bendirdjian E, Aradi J. (2005) Inhibition of human telomerase by oligonucleotide chimeras, composed of an antisense moiety and a chemically modified homo-oligonucleotide. FEBS Lett. 579, 1411-16.
2004
23.Tóth G, Schlammadinger J, Aradi J, Antal-Szalmas P, Sipka S, Balázs M, Kovács P, Fehér Z, Falus A. (2004) A DNA uptake-stimulating protein increases the antiproliferative effect of c-myb antisense oligonucleotide on leukemic cells. Cell Biol Int. 28, 817-23.
22. Chadha KC, Dembinski WE, Dunn CB, Aradi J, Bardos TJ, Dunn JA, Ambrus JL Sr. (2004) Effect of increasing thiolation of the polycytidylic acid strand of poly I:poly C on the alpha, beta and gamma interferon-inducing properties, antiviral and antiproliferative activities. Antiviral Res. 64, 171-7.
21. Bene L, Bodnár A, Damjanovich S, Vamosi G, Bacso z, Aradi J, Berta A, Damjanovich J. (2004) Membrane topography of HLA I, HLA II, and ICAM-1 is affected by IFN-gamma in lipid rafts of uveal melanomas. Biochem Biophys Res Commun. 322, 678-83.
2002
20. Nagy Z, Wang Y, Ervin-Cohen RA, Aradi J, Monia B, Wang LH, Stepkowski SM, Rui H, Kirken RA. (2002) Interleukin-2 family cytokines stimulate phosphorylation of the Pro-Ser-Pro motif of Stat5 transcription factors in human T cells: resistance to suppression of multiple serine kinase pathways. J Leukoc Biol. 72, 819-28.
2001
19. Szatmari I, Aradi J. (2001) Telomeric repeat amplification, without shortening or lengthening of the telomerase products: a method to analyze the processivity of telomerase enzyme. Nucleic Acids Res. 29, E3.
2000
18. Szatmari I, Tőkés S, Dunn CB, Bardos TJ, Aradi J. (2000) Modified telomeric repeat amplification protocol: a quantitative radioactive assay for telomerase without using electrophoresis. Anal Biochem. 282, 80-8.
1996
17. Tőkés S, Aradi J. (1996) (s4dU)35: a novel, highly potent oligonucleotides inhibitor of the human immunodeficiency virus type-1 reverse transcriptase. FEBS Lett. 396, 43-6.
1995
16. Tőkés S, Aradi J. (1995) Inhibition of Moloney murine leukemia virus reverse transcriptase by partially 5-thiolated polyuridylic acid. Biochem Biophys Acta-Gene Structure Exp. 1261, 115-20.
1992
15. Balajthy Z, Aradi J, Kiss IT, Elődi P. (1992) Synthesis and functional evaluation of a peptide derivative of 1-beta-D-arabinofuranosylcytosine. J. Med. Chem. 35, 3344-3349.
1985
14. Ho YK, Reddy VAR, Aradi J, Minowada J, Bardos TJ. (1985) Inhibition of DNA polymerase alpha from leukemic and normal human cells by partially thiolated human deoxyribonucleic acids. Cancer Biochem Biophys. 7, 85-94.
13. Aradi J, Ho YK. (1985) Antitemplate effect of polynucleotides and their hybrid complexes. Cancer Biochem Biophys. 7, 349-359.
1982
12. Aradi J, Zsindely A, Kiss Á, Szabolcs M, Schablik M. (1982) Separation of Neurospora crassa myo-inositol-1-phosphate synthase from glucose-6-phosphate dehydrogenase by affinity chromatography. Preparative Biochem. (1982) 12, 137-151.
1980
11. Ho YK, Aradi J, Bardos TJ. (1980) Enzymatic synthesis of polyuridylic acid containing modified bases. Nucleic Acids Res. 8, 3175-91.
1979
10. Ho Y-K, Fiel RJ, Aradi J, Bardos TJ. (1979) Structural characterization of partially thiolated poly(cytidylic acid).Biochemistry. 18, 5630-5.
9. Zsindely A, Szabolcs M, Kávai M, Schablik M, Aradi J, Szabó G. (1979) Demonstration of myo-inositol-1-phosphate synthase and its assumed defective variant in various Neurospora crassa strains by immunological methods. Acta Biol Acad Sci Hung. 30, 141-9.
1978
8. Aradi J, Schablik M, Zsindely A, Csongor J, Szabó G (1978) Factors influencing the uptake of DNA by Neurospora crassa.Acta Biochim. Biophys. Acad. Sci. Hung. 13, 259-67.
1977
7. Zsindely A, Szabolcs M, Aradi J, Schablik M, Kiss Á, Szabó G. (1977) Investigations on myo-inositol-1-phosphate synthase from the wild type and the inositol-dependent mutant of Neurospora crassa. Acta. Biol. Acad. Sci. Hung. 28, 281-90.
6. Schablik M, Szabolcs M, Kiss Á, Aradi J, Zsindely A. Szabó G. (1977) Conditions of transformation by DNA of Neurospora crassa. Acta Biol. Acad. Sci. Hung. 28, 273-9.
1975
5. Bardos TJ, Aradi J, Ho Y-K, Kalman TI. (1975) Biochemical properties of 5-sulfur-substituted pyrimidine nucleosides and nucleotides. Ann. New York Acad Sci. 255, 522-31.
4. Sipka S, Fésüs L, Aradi J, Deseő G, Szilágyi T. (1975) Local effect of single stranded polyadenylic acid on passive cutaneous anaphylaxis in mice. Agents and Actions 5, 272-3.
1973
3. Sipka S, Aradi J, Szilágyi T. (1973) Effect of cyclic AMP and polyadenylic acid treatment on the delayed hypersensitivity of guinea pigs. Acta Microbiol Acad Sci Hung. 20, 167-70.
1971
2. Aradi J, Zsindely A, Hauck M, Tankó B. (1971) Preparation of ribo-oligonucleotides from homopolymers. Acta Biologica Debrecina IX, 13-17.
1970
1. Zsindely A, Aradi J, Tankó B. (1970) On the mechanism of the degradation of nucleic acids by periodate oxidation. Acta Biochim Biophys Acad Sci Hung. 5, 285-293.
Patents:
1. New application of mononucleotides and nucleosides for therapeutic use. Hungarian Patent P 0600042 (2006).
2. Oligonucleotide chimeras as reverse transcriptase or telomerase inhibitors. Hungarian Patent P 05 00154. (2005).
3. Utilization of oligonucleotides with 4-thio-deoxyurydilates to label cell surface reducing molecules and lipid rafts. Hungarian Patent P 0301288. (2003).
4. Method for preparation of horse radish peroxidase. Hungarian Patent P 0203352/7 (2002).
5. Methods for inhibiting/treating HIV infections and AIDS related symptoms. PCT US, D11-027. (2001).
6. Sulphur containing oligo- and polynucleotides- with therapeutic and curative effect against HIV infection. Hungarian Patent P 9800777 (1998).
7. Anti-HIV activity of medicinal plant extract and its compounds with therapeutic and curative effect. PCT HU 97000 15 (1997).
8. Procedure for preparation of natural phospholipid containing product with respiration correcting activity. Hungarian Patent 209 414 (1992).
9. Procedure for preparation of food supplement with regenerating activity. Hungarian Patent 204 681 (1990).