Equipment

Mass spectrometers

  • Applied Biosystems Voyager-DE PRO MALDI-TOF for accurate molecular weight determination and peptide mass fingerprinting
 
  • Applied Biosystems 4000 QTRAP equipped with Turbo V and NanoSpray electronspray ion sources a high performance hybrid triple quadrupole/linear ion trap LC/MS/MS mass spectrometer suitable for protein identification, post-translational modification detection and protein quantitation

HPLC

  • Proxeon Easy nLC II (Bruker) nano LC for sample fractionation
  • Agilent 1100 nano LC for chromatographic separations

Luminex-based multiplex analysis

  • Bioplex system (BioRad)

Equipments for electrophoresis

  • BioRad IEF cell suitable for 7 cm, 11 cm, 17 cm and 24 cm strip analysis
 
  • BioRad Mini-PROTEAN Tetra Cell
 
  • BioRad PROTEAN II xi XL Cells
 
  • BioRad PROTEAN Plus Multi-Casting Chamber
 
  • BioRad PROTEAN Plus Dodeca Cell
 
  • BioRad PharosFX Plus System for gel documentation

Softwares

  • ProteinPilot 4.5 (ABSciex) for protein identification and quantifiction
  • Delta2D (Decodon) for accurate 2DE gel image analysis
  • PDQuest (BioRad) for 2DE gel image analysis
  • QuantityOne (BioRad) for 1D gel image analysis
  • MRMPilot (ABSciex) for SRM transition design
  • MultiQuant (ABSciex) for SRM data analyisis
  • Skyline (MacCoss Lab) for SRM transition design, optimization, data analysis and data publication (Panorama)


Ultracentrifuge

  • Kontron ultracentrifuge
  • Sorvall WX Ultra (Thermo Scientific) ultracentrifuge  
  • List of available rotors: 
 

Name

Max. RPM

1

Beckman JS-13.1

13000

2

Beckman JS-7.5

7 500

3

Kontron Type 50

50 000

4

Kontron TYP TFT 45.94

45 000

5

Kontron Type 35

35 000

6

Kontron SW 41 TI

41 000

7

Kontron SW 27

27 000

8

Kontron SW 55 TI

55 000

9

Kontron Type 50.4 TI

50 000

10

Kontron Type 19

19 000

11

Kontron TFT 50.38 Ti

50 000