Office address:

Laboratory Animal Core Facility

Department of Biochemistry and Molecular Biology,

Research Center for Molecular Medicine

Medical and Health Science Center, University of Debrecen, Hungary

Life Science Building, Nagyerdei krt. 98. Debrecen, Hungary H-4012

Phone: +36-52-416 432/62335 (office), 65234 (lab), 62325(MD), 62326 (SPF)

Fax: +36-52-314 989

e-mail:  This email address is being protected from spambots. You need JavaScript enabled to view it.  or  This email address is being protected from spambots. You need JavaScript enabled to view it.

Web: http://bmbi.med.unideb.hu/joomla15/index.php/en/core-facilities

1. Mission

The core facility was founded in 2005 to provide a professional background of animal experiments and promote best practices of humane care, use and management of animals involved in medical research at the Debrecen University.

We advance the development and acceptance of high standards for the responsible use of laboratory animals and accurate and reproducible animal experiments.

The Laboratory Animal Core Facility is administratively responsible for all animal care resources and the purchase and care of all laboratory animals in the Life Science Centre.

2. Housing conditions and laboratory capabilities

The facility is located on the Debrecen University campus as a part of the Life Science Centre. Suitable housing conditions are provided for a wide variety of outbred, inbred and genetically modified laboratory animals as mice, rats, guinea pigs and invertebrate organisms.

The Laboratory Animal Core Facility operates a clinical pathology laboratory for the purpose of basic disease surveillance and diagnosis as well as serving as a research resource to investigators. The laboratory capabilities include histology, immunocytochemsitry, clinical chemistry, chromatography, bacteriology.

3. Welfare and quality control

Specialized animal housing spaces include a quarantine suite, infectious biohazard containment suites. All animal rooms and the operation rooms are ventilated with HEPA filtered air. Our technologies see the requirements of FELASA recommendations and DIN EN ISO 9001 standards.

4. Members of the facility

Management

Supervisor

Zsuzsanna Szondy, MD, DSc

Professor of the Faculty of Medicine

Facility manager

Attila Pap, MSc

Biologist

Advisors

Árpád Bacsadi DVM

Pathologist

Péter Keserű DVM

Veterinarian

Judit Szabó MD, PhD

Bacteriologist 

Technicians: 

Sándor Sipos

Certified laboratory animal technician (2012-)

A2 level animal technician (2007-)

A1 level animal technician (2005-2007)

Certified technician of laboratory chemistry (1972-) 

János Tóth

Certified laboratory animal technician (2012-)

A1 level animal technician (2006-)

Certified medical technician (1983-)

Viktória Kurucz

A1 level animal technician (2012-)

Certified medical assistant (1998-)

Szabolcs Szalóczi

A1 level animal technician (2012-)

HEALTH MONITORING PROGRAMME

Animals are housed separately by species and, when possible, by source and or microbiological status. All rodent colonies are housed and maintained under minimal specific pathogen free (SPF) conditions. In our SPF definition we are negative for the following animal pathogens:

Diagnostic laboratories:

1. AnLab LTD, Videnska 1083, 142 20 Prague 4, Czech Republic, Phone: +420 261711667
2. BioDoc, Feodor-Lynen-Strasse 23. D-30625 Hannover, Germany, Phone: +49 511-548885
3. MGSzH Állategészségügyi Diagnosztikai Igazgatóság, 4031 Debrecen, Bornemissza u. 3-5., T:+36-52-418-133

AIR STERILITY

The animal rooms are ventilated with sterile filtered positive pressured (+10%) air. The exchange rate is 13.000 m3 air/hour (with tenfold ventilation). The sterility of air flow is tested monthly by sedimentation test and cultivation of sedimented bacteria.

LABORATORY ANIMAL STRAINS

MOUSE STRAINS

OUTBRED STRAINS

1. NMRI

INBRED STRAINS

1. BALB/c

2. C57 BL/ 6J

3. 129Sv

GENETICALLY MODIFIED STRAINS

1. Adora2 (-) (Tm)

2. Adora3 (-) (Tm)

3. Boy6J

4. CD11cCre (Tg)

5. Dicer (fl)/ LysMCre (Tm,Tg)

6. Nur77 (-) (Tm)

7. PARP1 (-) (Tm)

8. PARP2 (-) (Tm)

9. PPARg (fl) (Tm)

10. PPARg (fl)/ LysMCre (TmTg)

11. PPARg (fl)/ Sox2Cre (TmTg)

12. PSGL1 (-) (Tm)

13. RXRa (fl)/ RXRb (-) (Tm)

14. RXRa (fl)/ RXRb (-)/ LysCre (TmTg)

15. Sh3pxd2b (-) (Tm)

16. STAT1 (-) (Tm)

17. STAT6 (-) (Tm)

18. TGM2 (-) (Tm)

IMMUNODEFICIENT STRAINS

1. SCID

RAT

Wistar white

GUINEA PIG

Hartley

We do not maintain rat and guinea pig strains in the animal house, but we are able to do this. If somebody wants to use rats or guinea pigs, we could order the animals from proper vendors.

Price list (from 1st January 2014):

Animal maintenance (cage/day)

Pregnant female (for embrio isolation)

Maintenance of breeding cages

Animals for experiments from our own breeding colonies

Services

Other orders, demands, experiments according to customized agreement.

This Core Facility is associated with the Stem Cell Differentiation Laboratory (Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen).

 Locations and Contact Information:

H-4032, Debrecen, Egyetem ter 1. Life Science Building (LSB)
Location of FACS aria III: LSB 3.029
Location of FACS Calibur: LSB 3.043

 István Szatmári, PhD
Core Facility Supervisor
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
Phone: +36-52/416-432/65527

Pál Botó, PhD student
Core Facility Manager
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
Phone: +36-52/416-432/64593

 Services

- assisted and unassisted cell sorting, using BD FACS Aria III cell sorter
- unassisted analysis available 24/7, using BD FACS Calibur and BD FACS Aria III
- extensive training on FACS Aria III
- consultation on experimental design and data analysis

Instruments

BD FACS Aria III

Cell sorter with all available 6 lasers, running BD FACS Diva 6.1 on Windows XP
Available lasers: 375nm, 405nm, 445nm, 488nm, 561nm, 633nm
Multicolor flow cytometric analysis: 18 fluorescence parameters can be simultaneously monitored
Capable of 2 and 4 way sorting, plate sorting, sorting onto slide.

BD FACS AriaService Fees

Users from UD: 2000 HUF/h
Users outside of UD: 5000 HUF/h

The calculation of the service charge is based on the online booking.

BD FACS Calibur

FACS Calibur is equipped with two lasers, allowing for excitation at 488 nm and 633 nm.
Four channels can be set for fluorescent parameters. CellQuest software (Mac OS X) is used for sample acquisition and data analysis.

BD FACS AriaService Fees

Users from UD: 1000 HUF/h
Users outside of UD: 2500 HUF/h

The calculation of the service charge is based on the online booking.

Instruments booking

Online reservation is required for use of our services.
Reservation of our instruments is available through our Calendar after registration.
Editing or deleting your booking is available only at least 30 min before your appointment.

Go to the calendar for online reservation

Policies and guidelines (FACS Aria III)

The FACS Aria III can be operated by trained users, all new users must participate on a two-day training program.
The FACS Aria III cell sorter can be used 24/7. However, facility staff is only available during normal university work hours (8:00-16:00) for help.

Fluidics Startup:

While last user of the week has to perform Fluidics Shutdown (see below), first user of next week has to perform Fluidics Startup.

Daily cleaning:

After your analysis is completed, please follow the protocol:
- 5 min FACS Clean, - 5 min FACS Rinse, - 5 min DW

Daily Shutdown or “End of the day” cleaning:

Last user of the day has to perform according to the following protocol:
Daily cleaning + 2 sets of Clean Flow Cell method (FACS Clean, FACS Rinse, DW)

Weekly Shutdown:

Last user of the week (usually on Friday) has to perform “Fluidics Shutdown”.

Data management and storage:

It is the responsibility of all registered users to copy their data to a secondary computer device. You can bring your flash drive to transfer data.
After copying your data please leave only.

Useful links

http://nlab.med.unideb.hu/~calendar/index.php

https://www.bdbiosciences.com/

http://www.ebioscience.com/