Curriculum vitae

• M.S.: Chemistry, Kossuth Lajos University of Sciences, Hungary, 1996
• Ph.D.: Molecular and cell biology, University Medical School of Debrecen, Hungary, 1999

• 1996-1999: Ph.D. student, Department of Biochemistry, University Medical School of Debrecen, Hungary
• 1998: TEMPUS-JEP Fellowship in Department of Biochemistry, Medical University of Koeln, Germany
• 1998: fall fellowship in laboratory of Terry D. Copeland, Proteinn Chemistry section, ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Frederick, USA
• 2000: summer fellowship in laboratory of Irene T. Weber, Thomas Jefferson University, Jefferson Cancer Institute, Philadelphia, USA
• 2001: fall fellowship in laboratoy of Irene T. Weber, Georgia State University, Atlanta, USA
• 2002: spring fellowship in laboratory of Irene T. Weber, Georgia State University, Atlanta, USA
• 2003-2004: postdoctoral fellowship in laboratory of Irene T. Weber, Georgia State University, Atlanta, USA
• 2000-2006: Assistant Lecturer, Department of Biochemistry and Molecular Biology, University of Debrecen, Hungary
• 2006-present: Senior Lecturer, Department of Biochemistry and Molecular Biology, University of Debrecen, Hungary

• 1996 Prize from Society of Hungarian Chemists for thesis of M.Sc.

• Hungarian Society of Biochemistry
• Hungarian Society of Proteomics

• Sperka T, Boross P, Eizert H, Tozser J, Bagossi P. (2006) Effect of mutations on the dimer stability and the pH optimum of the human foamy virus protease. Protein Eng. Des. Sel. 19(8):369-75.
• Fang B, Boross PI, Tozser J, Weber IT. (2006) Structural and kinetic analysis of caspase-3 reveals role for s5 binding site in substrate recognition. J. Mol. Biol. 360(3):654-66.
• Feher A, Boross P, Sperka T, Miklossy G, Kadas J, Bagossi P, Oroszlan S, Weber IT, Tozser J. (2006) Characterization of the murine leukemia virus protease and its comparison with the human immunodeficiency virus type 1 protease. J. Gen. Virol. 87(Pt 5):1321-30
• Liu F, Kovalevsky AY, Louis JM, Boross PI, Wang YF, Harrison RW, Weber IT. (2006) Mechanism of drug resistance revealed by the crystal structure of the unliganded HIV-1 protease with F53L mutation. J. Mol.Biol. 358(5):1191-9.
• Kovalevsky AY, Tie Y, Liu F, Boross PI, Wang YF, Leshchenko S, Ghosh AK, Harrison RW, Weber IT. (2006) Effectiveness of nonpeptide clinical inhibitor TMC-114 on HIV-1 protease with highly drug resistant mutations D30N, I50V, and L90M. J.Med.Chem. 49(4):1379-87
• Fengling, L., Boross, P., Wang, Y.F., Tozser, J., Louis, J.M., Harrison R.W., Weber, I.T. (2005) Distinct structural changes in high resulotion crystal structures of HIV-1 protease with drug resistant mutations, L24I, I50V and G73S. Journal of Molecular Biology 354(4): 789-800
• Vana, M.L., Boross, P., Weber, I.T., Colman, D., Barklis, E., Leis, J. (2005) Mutations affecting cleavage at the p10-Capsid protease cleavage site block Rous Sarcoma Virus replication. Retrovirology 27,2:58
• Boross, P, Tozser, J., Bagossi, P. (2005) Improved purification for wild-type and mutant human foamy virus proteases. Protein Expression and Purification 46(2):343-7
• Tie, Y., Boross, P., Wang, Y.F., Gaddis, L., Liu, F., Chen, F., Tozser, J., Harrison. R.W., Weber, I.T. (2005) Molecular basis for substrate recognition and drug resistance from 1.1-1.6 A resolution crystal structures of HIV-1 protease mutants with substrate analogs. FEBS Journal 272 (20):5265-77
• Bagossi, P., Sperka, T., Feher, A., Kadas, J., Zahuczky, G., Miklossy, G., Boross, P., Tozser, J.(2005) Amino acid preferences for a critical substrate binding subsite of retroviral proteases in type 1 cleavage sites. J. Virol., 79, 4213-4218.
• Bagossi, P., Kadas, J., Miklossy, G., Boross, P., Weber, I.T., Tozser J. (2004) Development of a microtiter plate fluorescent assay for inhibition studies on the HTLV-1 and HIV-1 proteinases. J. Virol. Methods, 119, 87-93.
• Kadas, J., Weber, I.T., Bagossi, P., Miklossy, G., Boross, P., Tozser J. (2004) Alteration of the specificity of human T-cell leukemia virus type-1 protease. J. Biol. Chem., 279, 27148-27157.
• Feher, A., Boross, P., Sperka, T., Oroszlan, S. and Tozser J. (2004) Expression of the murine leukemia virus protease in fusion with maltose binding protein in Escherichia Coli. Protein Exp. Purif. 35, 62-68.
• Mahalingam, B., Wang, I.F., Boross, P., Tozser, J., Louis, J.M., Harrison, R.W. and Weber, I.T. (2004) Crystal structures of HIV protease V82A and L90M mutants reveal changes in indinavir binding site. Eur. J. Biochem, 271, 1516-1524.
• Tie, Y., Boross, P.I., Wang, Y.F., Gaddis, L., Hussain, A.K., Leshchenko, S., Ghosh, A.K., Louis, J.M., Harrison, R.W., Weber, I.T. (2004) High resolution crystal structures of HIV-1 protease with a potent non-peptide inhibitor (UIC-94017) active against multi-drug-resistant clinical strains. J. Mol. Biol. 338, 341-352
• Tozser, J., Shulenin, S., Kadas, J., Boross, P., Bagossi, P., Copeland, T.D., Nair, B.C., Sarngadharan, M.G., Oroszlan, S. (2003) Human immunodeficiency virus type 1 capsid protein is a substrate of the retroviral proteinase while integrase is resistant toward proteolysis. Virology, 310, 16-23.
• Koh, Y., Nakata, H., Maeda, K., Ogata, H., Bilcer, G., Devasamudram, T., Kincaid, J.F., Boross, P., Wang, Y.F., Tie, Y., Volarath, P., Gaddis, L., Harrison, R.W., Weber, I.T., Ghosh, A.K. and Mitsuya, H.(2003) Novel bis-tetrahydrofuranylurethane-containing nonpeptidic protease inhibitor (PI) UIC-94017 (TMC114) with potent activity against multi-PI-resistant human immunodeficiency virus in vitro. Antimicrob Agents Chemother. 47, 3123-3129
• Mahalingam, B., Boross, P., Wang, Z.F., Louis, J.M., Fischer, C., Tozser, J., Harrison, R.W. and Weber, I.T. (2002) Combining mutations in HIV-1 protease to understand mechanisms of resistance. Proteins, 48, 107-116
• Feher, A., Weber, I.T., Bagossi, P., Boross, P., Mahalingam, B., Louis, J.M., Copeland, T.D., Torshin, I.Y., Harrison, R.W., Oroszlan, S. and Tozser, J. (2002) Effect of sequence polymorphism and drug resistant mutations at two HIV-1 Gag cleavage sites on their proteolytic susceptibility. Eur. J. Biochem. 269, 4114-4120
• Zahuczky, G., Boross, P., Bagossi, P., Emri, G., Copeland, T.D., Oroszlan, S., Louis, J.M., Tozser, J. (2000) Cloning of the bovine leukemia virus proteinase in Escherichia coli and comparison of its specificity to that of human T-cell leukemia virus proteinase. Biochimica et Biophysica Acta - Protein structure and molecular enzymology, 1478, 1-8.
• Boross, P., Bagossi P., Copeland, T.D., Oroszlan, S., Louis, J.M., Tozser, J. (1999) Effect of substrate residues on the P2 ' preference of retroviral proteinases. European Journal of Biochemistry, 264, 921-929.
• Tozser, J., Bagossi, P., Boross, P., Louis, J.M., Majerova, E., Oroszlan, S., Copeland, T.D. (1999) Effect of serine and tyrosine phosphorylation on retroviral proteinase substrates. European Journal of Biochemistry, 265, 423-429.
• Fenyofalvi, G., Bagossi, P., Copeland, T.D., Oroszlan, S., Boross, P., Tozser, J. (1999) Expression and characterization of human foamy virus proteinase. FEBS Letters, 462, 397-401.
• Takacs L, Boross P, Tozser J, Modis L Jr, Toth G and Berta A (1998) Transforming growth factor-beta induced protein, betaIG-H3, is present in degraded form and altered localization in lattice corneal dystrophy type I. Exp. Eye Res. 66 (6): 739-45.
• Nemes Z Jr, Adany R, Balazs M, Boross P and Fesus L (1997) Identification of cytoplasmic actin as an abundant glutaminyl substrate for tissue transglutaminase in HL-60 and U937 cells undergoing apoptosis. J. Biol Chem 272 (33): 20577-83.
• Bogati MS, Pocsi I, Maticsek J, Boross P, Tozser J and Szentirmai A (1996) NADP-specific glutamate dehydrogenase of Penicillium chrysogenum has a homohexamer structure. J. Basic Microbiol 36 (5): 371-5.
• Nogrady N, Pocsi I, Katona E, Jeney V, Boross P, Tozser J, Fachet J and Szentirmai A (1996) Soluble cell-bound and extracellular cyclodextrin glycosyltransferases of Bacillus macerans show identical enzymological characteristics and antigenicity. J. Basic Microbiol 36 (5): 335-40.