KiDet
Type of calculation?
b) K
m
, V
max
determination of a substrate
Type of microtiter plate?
a) microtiter plate type 1 (transparent)
b) microtiter plate type 2 (solid white)
Which substrate?
a) substrate: IB354
b) substrate: IB404
c) substrate: IB405
d) substrate: IB406
e) substrate: IB407
f) substrate: IB524
g) substrate: IB525
h) substrate: IB526
z) substrate: unknown (average)
Type of input data?
a) time [min:sec] vs. absorbance [uOD]
Data:
Enzyme concentration [nM]?
Substrate concentrations [nM]?
MikroOD to nM conversion factor (ODconv)?